Welcome to anti-CRISPRdb

CRISPR is a tool that is widely used for gene editing. However, unexpected off-target effects may occur as a result of long-term nuclease activity. Anti-CRISPR proteins, which are powerful molecules that inhibit the CRISPR-Cas system, may have the potential to promote better utilization of the CRISPR system in gene editing, especially for gene therapy. Additionally, more in-depth research on these proteins would help researchers to better understand the co-evolution of bacteria and phages. Therefore, it is necessary to collect and integrate data on various types of anti-CRISPR proteins. Data on these proteins were manually gathered through text mining and data screening of the literatures. We then constructed anti-CRISPRdb: this is the first online resource for organizing these proteins.

Anti-CRISPRdb resource acts as an online resource to organize the anti-CRISPR proteins. It enables easy browsing of the anti-CRISPRs that have been published. It contains the available protein sequences, DNA sequences, coding regions, source organisms, taxonomy, virulence, and protein interactors and the corresponding three-dimensional structures. The first version of anti-CRISPRdb currently contains 432 anti-CRISPR proteins and 106 non-redundant ones tested by experimental and bioinformatics methods. These proteins can be divided into the following five categories: anti-CRISPR proteins with anti-I-F activity, those with anti-I-E activity, those with anti-II-A activity, those with anti-II-C activity, and those with anti-IV-B activity.

Data Update - May 2018:
1. We added two new subtype Acrs: AcrIIA5 (PMID: 28785032), AcrID1 (PMID: 29507349). They can be downloaded Here. Please note that data is not contained in our blast database. We will update our blast database in next version.
2. Self-targeting of a genome have been used as a maker by investigators to screen potential bacteria that bear Acrs. Therefore, to perform the annotation and classification task of Acrs, it is necessary to confirm the CRISPR-Cas locus and its (sub)type that a bacterium maintain. We proposed a web-based server, CasLocusAnno to annotate Cas locus and (sub)type based on a previous work.
3. An updated version of Anti-CRISPRdb (anti-CRISPRdb2) are avaiable now.
4. Release a standlone script, AcrDetector, to determine anti-CRISPR proteins in the whole genome-scale. Download here.

Citation:Chuan Dong, Ge-Fei Hao, Hong-Li Hua, Shuo Liu, Abraham Alemayehu Labena, Guoshi Chai, Jian Huang, Nini Rao, Feng-Biao Guo; Anti-CRISPRdb: a comprehensive online resource for anti-CRISPR proteins, Nucleic Acids Research, gkx835, https://doi.org/10.1093/nar/gkx835